Effect of two volatile oils extracted from Syzygium aromaticum, Mentha longifolia, and imidacloprid on Lucilia cuprina (Diptera: Calliphoridae)

Background: The L3 of Lucilia cuprina grow and feed on the living flesh sheep. Besides, they cause cutaneous myiasis. Herbal medicine showed potential efficacy against several pathogens as well insecticidal effects. Objectives: present work aims to investigate effect two volatile oils, Syzygium aromaticum, Mentha longifolia chemical neonicotinoid, imidacloprid (IMI), developmental stages L. cuprina. Material Methods: Early were exposed five concentrations three selected compounds under investigation. procedures replicated four times for each concentration, 25 larvae used replicate. Average larval mortality rates subjected probit model analysis calculating LC25, LC50, LC90. Acetylcholine esterase (Ach E) activity was measured using acetylcholine bromide Br) substrate because testing have anti -cholinesterase activity. Results: It observed that oil LC50 M. (3.35%) gave higher third instar compared with produced by S. aromaticum (6.06%). IMI most effective larvicidal agent 0.000853%. Both oils induced complete disintegration in epicuticle epidermis integument, extensive damage epithelial cells midgut. Treatments both deformed adult flies, significant highest pupal after treatment IMI, controls. AcH E insignificant reduction essential IMI. Conclusion: inhibitory biology, histology Ach suggest may be considered control agents myiasis causing fly